Nicotine‐free, nontransgenic tobacco ( Nicotiana tabacum l .) edited by CRISPR ‐Cas9
نویسندگان
چکیده
منابع مشابه
Coproporphyrinogenase in tobacco (Nicotiana tabacum L.).
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
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Plant suspension cells are interesting hosts for the heterologous production of pharmacological proteins such as antibodies. They have the advantage to facilitate the containment and the application of good manufacturing practices. Furthermore, antibodies can be secreted to the extracellular medium, which makes the purification steps much simpler. However, improvements are still to be made rega...
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The use of tobacco dates back to the ancient civilizations of the Americas, where it played a central role in religious occasions. The peoples smoked tobacco in cigars and pipes and chewed it with lime, for its pleasurable euphoriant effects. In the 16 th century, Europeans spread the use of tobacco in North America, while the Spanish bring it into Europe. In the 1559, Jean Nicot, the French am...
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BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new ...
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Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the ingestion of gluten proteins from wheat, barley and rye. The α-gliadin gene family of wheat contains four highly stimulatory peptides, of which the 33-mer is the main immunodominant peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent to the coding sequence...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2019
ISSN: 1467-7644,1467-7652
DOI: 10.1111/pbi.13193